Tissue Culture Process
![](https://www.terranovanurseries.com/wp-content/uploads/2017/11/cutting-hood.jpg)
Tissue Culture is a high-tech procedure enabling many plants to be propagated under sterile conditions.
![](https://www.terranovanurseries.com/wp-content/uploads/2017/11/pulmonaria-david-ward.jpg)
Stage I: We start with a specimen: Pulmonaria ‘David Ward’
![](https://www.terranovanurseries.com/wp-content/uploads/2017/11/stage-II-cut.jpg)
Stage I: The growth tip is “excised” (removed) and then sterilized in a bleach solution.
![](https://www.terranovanurseries.com/wp-content/uploads/2017/11/initiation-stage-I.jpg)
Stage I: This explant is then placed in a test tube with agar, nutrients, and a hormone to induce shoots.
![](https://www.terranovanurseries.com/wp-content/uploads/2017/11/stage-II.jpg)
Stage II: Shoots multiply in the shoot-media and are cut again and again at set intervals. The numbers increase geometrically until the desired amount is met.
![](https://www.terranovanurseries.com/wp-content/uploads/2017/11/lights.jpg)
Plants are grown at stages one and two in a climate controlled grow-room.
![](https://www.terranovanurseries.com/wp-content/uploads/2017/11/stageIII.jpg)
Stage III: When numbers are met the stage two explants are placed in a root inducing media.
![](https://www.terranovanurseries.com/wp-content/uploads/2017/11/transfer.jpg)
Stage IV: When roots are formed they can be put into soil. On transfer to soil, plants are considered Stage IV.
![](https://www.terranovanurseries.com/wp-content/uploads/2017/11/greenhouse.jpg)
Plants are greenhouse-weaned in even humidity, light, and temperature.
![](https://www.terranovanurseries.com/wp-content/uploads/2017/11/finish.jpg)
Flats are transferred to our growing house to be hardened-off, and set for shipping. The entire process can take from 3 months to a year.